FLUORESCENT PARTICLES

 

Fluorescence microscopy image of MF-FITC-particles
Fluorescence microscopy image of MF-FITC-particles

 

Fluorescence emission spectra of free FITC and MF-FITC particles
Fluorescence emission spectra of free FITC and MF-FITC particles

 

Confocal fluorescence microscopy image of an internally colored MF-ethidium bromide particle
Confocal fluorescence microscopy image of an internally colored MF-ethidium bromide particle

Novel manufacturing techniques developed and patented by microparticles GmbH allow the preparation of a wide variety of fluorescent microspheres ranging in size, matrix chemistry, type of fluorochrome, fluorescence intensity, and surface functional groups.
Some of the fluorochromes used in our new MF-fluorescent particles are:
  • 7-Amino-4-methylcoumarin (AMC)
    (blue fluorescence, Excitation/Emission =360/429 nm)
  • FITC
    (green fluorescence, Excitation/Emission =506/529 nm)
  • Acridin Orange
    (green fluorescence, Excitation/Emission = 510/522 nm)
  • Ethidium Bromide
    (orange fluorescence, Excitation/Emission = 302 (505)/602 nm)
  • Rhodamine B
    (orange fluorescence, Excitation/Emission = 560/584 nm)
  • Nile Blue A
    (red fluorescence, Excitation/Emission = 636/686 nm)
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These fluorescent particles have the following advantages:
  • an extremely narrow size distribution with a wide diameter range from 0,3 to 15 µm
  • fluorochromes are internally incorporated with a uniform distribution
  • intense color and fluorescence
  • high stability in organic solvents
  • available as aqueous suspensions (2,5% solids) or as free-flowing, dry powders
  • available as functionalized particles with a high density of functional groups
    •

Microparticles GmbH also offers multifluorescent polymer particles, dyed with up to three different fluorochromes (for instance: FITC-Rhodamine B-Nile Blue A).
The fluorescent particles can be used as standards (e.g. in Flow Cytometry, Confocal Laser Scanning Microscopy, Light Scattering Instruments) as well as tracers in environmental science, flow measurements in gases and liquids like Laser Doppler Anemometry (LDA), Particle Dynamics Analysis (PDA), and Particle Image Velocimetry (PIV).

Fluorescent particles:
(Click "Table"-buttons to view the complete particle spectrum or enter your desired particle diameter in µm)

  • AMC-labeled MF-Polymer Particles in the Size Region 1-10 µm
    		•
  • FITC-labeled MF-Polymer Particles in the Size Region 500 nm-10 µm
    		•
  • Acridine Orange-labeled MF-Polymer Particles in the Size Region 1-10 µm
    		•
  • Ethidium Bromide-labeled MF-Polymer Particles in the Size Region 1-10 µm
    		•
  • Rhodamine B-labeled MF-Polymer Particles in the Size Region 400 nm-12 µm
    		•
  • Nile Blue A-labeled MF-Polymer Particles in the Size Region 500 nm-10 µm
    		•

    Pack size:

  • aqueous dispersions (25 mg/ml): 5 ml, 10 ml, 15 ml
  • powder

    • -> Inquiries for other dyes or special multiple coupling of up to three dyes are welcome.

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